Antagonistic effect of Pseudomonas fluorescens isolated from soil of Doon Valley (Dehradun–India) on certain phyto-pathogenic fungi

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Rajendra Prasad, Harish Chandra, Bijendra Kumar Sinha, Jatin Srivastava

 Department of Forestry, Uttaranchal University, Dehradun, Uttarakhand India

 High Altitude Plant Physiology Research Center, H.N.B. Garhwal University, Srinagar – 246174, Uttarakhand, India

Department of Microbiology, Gayatri College of  Biomedical Sciences, Ballupur Chowk, Dehradun, Uttarakhand, India.

Department of Environmental Sciences, Global Environment Engineering Service, Lucknow – 226016, UP,  India.

In line with the major biological control measures to control the soil borne fungal infection in crop plants were worked out in District Dehradun (Uttarakhand) India. Pseudomonas fluorescens was isolated from soil samples collected from various places of Dehradun district and was investigated for antagonistic activity against native fungal phytopathogens viz., Alternaria alternata, Aspergillus flavus, Aspergillus niger, Erysiphe cruciferarum, Fusarium oxysporum by dual culture method. Maximum antagonistic effect of P. fluorescens was observed against F. oxysporum (73.24 ±2.40 %) followed by A. alternata (51.20 ± 0.12 %), A. flavus (37.85 ± 0.83%), E. cruciferarum (37.12 ± 0.66%) and least by A. niger (32.13 ± 1.77 %). Relative percentage of germination of spores of phyto-pathogen in culture filtrate of P. fluorescens was also investigated against these phyto-pathogens and it was found correlated with the antagonistic effect. Present study shows the prevalence of natural biological control on the phyto-pathogenic fungi.

 

Keywords: Pseudomonas fluorescens; phyto-pathogenic fungi; spore germination; antagonism; dual culture method.

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