Antibacterial activity of Root and Leaf Extracts of Carissa spinarum against Methicillin-resistant Staphylococcus aureus (MRSA) and other Clinically Pathogenic bacterial strains

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Vinod kumar, Vijay Joshi, and Ravinder Singh

Department of Chemistry, Uttaranchal University, Dehradun, Uttarakhand, India

 

Root and Leaf Extracts of Carissa spinarum were evaluated against 15 isolates of methicillin-resistant S. aureus isolates and other Clinically Pathogenic bacterial strains. Antimicrobial activity of Carissa spinarum root and leaves extracts obtained with different solvents viz., water, methanol, ethanol, hexane, petroleum ether, chloroform and ethyl acetate was evaluated against some selected pathogenic microorganisms such as Proteus, Pseudomonas flouorescence, MRSA and Escherichia coli using disk diffusion method. Antimicrobial activity of root extract of ethyl acetate is high (25mm) and in leaf hexane extract has showed the highest activity (30mm). From the phytochemical analysis alkaloids, flavonoids, saponin, glycoside, tannins and steroid was found active constituents and is believed to be responsible for their antimicrobial  activities.

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