Antimicrobial activity of Rhizome Extract of Acorus calamus Against Different Micro-Organisms

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Vinod Kumar , Ravinder singh and Vijay joshi

Department of Life Science, Beehive P.G. College, Dehradun, Uttarakhand, India

Acorus calamus is a perrenial, semi-aquatic and smelly plant, found in both temperate and subtemperate zones. It has long been known for its medicinal value. The rhizome extract has good antimicrobial activity. Antimicrobial activity of Acorus calamus rhizome  extracts obtained with different solvents viz., water, methanol, ethanol, butanol, hexane, petroleum ether and ethyl acetate was evaluated. From the phytochemical analysis flavonoid, glycoside, saponin,  resin and steroid was found in the rhizome extract and is believed to be responsible for their antimicrobial  activities. Extract obtained with ethyl acetate and ethanol among other  were found to be highly effective. Rhizomes ethyl acetate and ethanol extracts exhibited pronounced antibacterial activity against MRSA  with diameter zone of inhibition ( 22-26mm) and antifungal activity against Aspergillus niger with diameter zone of inhibition (20mm). The minimum inhibitory concentration (MIC) of the rhizome extracts for antimicrobial activity was done by serial dilution viz., 100mg/500µl, 50mg/500µl, 25mg/500µl, 12.5mg/500µl and 6.25mg/500µl.

Keyword - Antimicrobial activity,  Rhizome, Extract,  Acorus calamus.

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