Chloroplast trnT-trnF gene constitutes an excellent marker to understand the polymorphism and evolution of Dioscorea species

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Joel Bleoue, Koffi K. Edmond, Djaman A. Joseph, Ama Lucie and Traore Allassane

1. UFR de Biosciences, Université Félix Houphouet-Boigny, 01 BP V4 Abidjan 01 Côte d’Ivoire

2. Laboratoire Central de Biotechnologies, 01 BP 1740 Abidjan 01, Côte d’Ivoire

The trnT-trnF chloroplast gene has been described as an excellent marker for demonstrating genetic diversity and understanding the evolution of plants through molecular methods such as polymerase chain reaction and phylogenetic studies. In fact, the trnT-trnF chloroplast gene exhibits a high degree of localized polymorphism within the spacers and the intron and this variability of the trnT-trnF gene allowed plants to diversify and evolve. Understanding the variability of the structure of the trnT-trnF gene and the evolution of Dioscorea species through this study will assist plant development. Our PCR et sequencing results revealed considerable length variation of trnT-L, trnL and trnL-F between the Dioscorea sp of Cote D’Ivoire and the other Dioscorea sp. We also found that a part of the trnT-L gene of Dioscorea sp of Cote D’Ivoire has been deleted whereas the trnL intron has undergone an insertion.

 

Keywords: Dioscorea sp.; Chloroplast DNA, trnT-L; trnL intron; trnL-F; Phylogenetic

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