Comparative analysis based on 2-methanesulfonylpyridine (MSP) in blood plasma of fresh water fish under the exposure of toxicants

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Vishal Rajput , Rabinder Negi, Richa Gaur

                                       Department of Biotechnology and Biochemistry Sardar Bhagwan Singh University. Uttarakhand

Present investigation was focused on evaluation of hair care products containing ZnPT only and ZnPT with EDTA. Toxicity comparison was based on MSP content in fresh water fish plasma under the exposure of shampoos containing ZnPT alone and a combination of ZnPT and EDTA. Blood samples were collected before treatment and at 0.5, 1, 2, 3, 4, 6, 8, 12, 16, 20 and 24 hrs after treatment. Plasma MSP concentrations were not measurable from 0.5 hrs 8 hrs after treatment with shampoo containing 1% ZnPT, and from 0.5 to 8 hrs after treatment with shampoo containing 1% ZnPT + 2% EDTA. Thereafter, MSP concentration kept elevating up to 24 hr in both groups. This composition is speculated to increase ZnPT absorption and the resulting increased toxicity in aquatic organisms.

Keywords: EDTA, Fresh water fish, Zinc Pyrithione, EDTA, Metabolite

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