Cytotoxic response of SMA-DMSO complex along with other test chemical on the HEPA RG and CHO cell line

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Avinash Singh, Chaiti Ganguli & R. K. Singh

                                Department of Biotechnology, CET, IILM – AHL, Greater Noida, UP, India

                                     CSIR-Central Drug Research Institute, Lucknow – UP, India

Safe, non-toxic and well accepted contraceptive has a great demand to control the increasing human population. In this connection, a lot of hormonal and non-hormonal contraceptives have been developed. SMA-DMSO complex is one of the remarkable break-through in the research of male contraceptive. Lot of researches has been made to prove the safe and non-toxic nature of this non hormonal polymeric compound. Present study demonstrates the non-cytotoxic nature of SMA-DMSO complex as compared to the DMSO and SDS (as negative control) and H2O2 (as positive control). The result shows that SMA-DMSO complex significantly decreases the MTT in both culture (CHO & HEPA RG). The concentrations of the test chemicals ranging between 2.5 to 10 µg were observed to decrease the % cytotoxicity in contrast to lower and higher concentration levels. SMA-DMSO complex has been observed to have least effect on the cellular viability.

Key words: Male contraceptive; SMA-DMSO, MTT assay; cytotoxicity; animal cell line

 

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