Determination of genetic relationship of some medicinally important Phyllanthus species by isozyme and nrITS-RFLP polymorphism markers

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Subhendu Bandyopadhyay and Sarmistha SenRaychaudhuri

                                  Department of Biophysics, Molecular Biology and Bioinformatics,University of Calcutta, Kolkata.

 Superoxidedismutase, Peroxidase and Esterase were used as biochemical markers to assess the genetic relationships between five species of the genus Phyllanthus. All three isozymes showed unique banding patterns for different species. A dendrogram was constructed depending upon these banding patterns, which assessed their genetic relationship properly. Nuclear ribosomal Internal Transcribed Spacer sequence based restriction fragment length polymorphism markers are also developed in this study. Among several restriction enzymes used in this study HpaII found to be the most helpful in distinguishing all the five species under study.

Key Words: Isozymes, Phyllanthus, Dendrogram, Nuclear ribosomal Internal Transcribed Spacer sequence (nr-ITS), restriction fragment length polymorphism (RFLP) and Genetic Relationships.

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