Bonny Aya Carole, Karou Tago Germain., Ngazoa Kakou Solange and Niamké Lamine Sébastien.

Biotechnology Laboratory of Biosciences departments, Félix Houphouët-Boigny University of Cocody, Abidjan,  Côte d’Ivoire.

Molecular Biology plateform of Pasteur Institute of Côte d’Ivoire, 01 P.O. Box 490 Abidjan 01, Côte d’Ivoire.

Laboratory of Biotechnology and Food Microbiology of Food Science and Technology department, University of Nangui Abrogoua, 02 P.O. Box 801 Abidjan 02, Côte d’Ivoire.

 Multidrug resistance is emerging in many Gram-negative bacteria like Salmonella spp. Plasmid encoding bla-Ctx-m enzymes represent an important sub-group of class A β-lactamases responsible for ESBL (Extended-spectrum β-lactamases) phenotype which is increasingly found in Enterobacteriaceae such as Salmonella spp. Molecular typing of ESBL-isolates has become more important for prevention of ESBL-producers dissemination in environment. bla Ctx-m genes were targeted using degenerated bla Ctx-m consensus primers and PCR amplified from Salmonella strains presenting particular features of multidrug resistance, isolated from chicken gizzards. bla Ctx-m sequences analysis in Salmonella isolates (Kentucky and Muenster), revealed similarities of 96 to 100 % of homology with nucleotides fragments encoding to enzymes type such as bla CTX-M-2,-5,-44,-59,-92,-97 and -131; bla NDM-1 and bla OXY. This identification could only been achieved by sequencing of the PCR-amplicons in other Enterobacteriaceae. PCR-based molecular typing method described here, enables a rapid and reliable molecular identification of bla genes. The principles used in this study could also be applied to any situation in which antimicrobial resistance genes would need to be sequenced.

Key words: Salmonella, bla Ctx-m genes, raw chicken gizzards, Côte d’Ivoire.