Algal Research and Bioenergy Laboratory, Department of Chemistry, Uttaranchal University,                                       Dehradun, Uttarakhand 248007, India

Microalgae-based biofuel generation is an encouraging alternative for the next generation renewable and sustainable biofuels. Microalgae biomass is a competent raw material for the generation of various biofuels including biodiesel, bioethanol, biomethane, and biohydrogen. Progress in the microalgae biofuel area desires abundant ecological conditions such as reasonable light, temperature, pH, and nutrients. Furthermore, the environmental conditions mentioned above are generally not noble with the beneficial conditions for generating biofuels. However, biochemical compositions, such as lipids and carbohydrates, can be influenced to increase the content under stress conditions of light, temperature, nutrients and salinity. In this paper, the impact of environmental stress factors is concise to improve microalgae biofuel generation by stimulating a significant increase in biochemical components.