Department of  Pharmaceutics, Vaagdevi Pharmacy College, Bollikunta, Warangal, Telangana, India

The plant extracts were screened for anti angiogenesis activity, anti fungal activity, anti oxidant and antibacterial activity against different species by the agar well diffusion assay. The ethanolic extract of M. oleifera were found active against both Gram-positive and Gram-negative bacteria. The antiangiogenic efficacy of M. oleifera leaf extract was examined in vivo in the chick chorioallantoic membrane (CAM) model by using fertilized eggs. Decreases in the density of the capillaries were assessed and scored. The results showed scores of 0.5 for 5 μg, score 2.0 for 10 μg extract have an inhibitory effect on angiogenesis. Extract of M. oleifera possessed potent antifungal activity against Aspergillus niger showing inhibition of 10 mm, Curvular ialunata 6mm and Aspergillus terreus 4mm. In case of anti oxidant activity studies, leaf extract exhibited the strongest DPPH inhibition activity (IC value of 19.46±2.71 μg/ mL) and showed better DPPH radical scavenging ability than ascorbic acid 21.36±2.31 μg/ mL).

Keywords: Moringa oleifera, Chorioallantoic membrane, Gram-positive and Gram-negative bacteria.