Immunohistochemistry based approach to study cross reactivity of certain commercially available antibodies with mammary tumor cell proteins of dog

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Swagatika Parida, Sanatan Majhi* , Voddu Suresh, Sidhartha Sankar Behera, Debiprasanna Das, Abinash Dutta

Department of Biotechnology, Utkal University, Bhubaneswar-751004, Odisha, India

A mammary tumor is a neoplasm in mammary gland. Immunohistochemical analysis of protein biomarkers like E-cadherin, Ki-67 and COX-2 etc. has a great importance in identifying the neoplastic cells. The current study aims to analyze the cross reactivity of the commercial antibodies against these proteins with Canine Mammary Tumor (CMT) tissue, which usually are used for detection of human mammary tumor. The present work was conducted with 20 clinical cases of surgically excised CMT using standard protocols.  The expression of E-cadherin, Ki-67 and COX-2 proteins were observed in cell membranes, nucleus and cytoplasm respectively in the positive CMT tissue. These commercial antibodies cross reacted with the biomarker proteins of CMT tissue. The results suggest that, these proteins might have some conserved regions in their corresponding genes, which needs further study to understand the phenomenon. These commercial antibodies can also be used for diagnosis and prognosis of CMT in the dog.

Keywords: Biomarker, canine mammary tumor, diagnosis, neoplasm, prognosis

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