Immunoscreening Of Lymphatic Filariid Brugiamalayi L3 Cdna Library Reveals Novel Vaccine Candidates

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Prashant Kumar Singh, Vishal K Soni, Mohd. Shahab, ShailjaMisra-Bhattacharya

Division of Parasitology, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, Uttar Pradesh 226031

 

Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, M.P

Immuno screening of cDNA library has been the most preferred choice to pick up desired gene from a pool of genome.We have identified three novel immunodominant cDNA clones, BmL3-thioredoxin, BmL3-hydrolase and BmL3-Cytochrome P450 by immunoscreening of a larval stage cDNA library of Brugiamalayi. Currently there are no adulticidal drugs or vaccines available against worms that are causative agents of lymphatic filariasis. The identified clones can be potent vaccine candidates for developing prophylactic agents against the human filarial parasite. Nucleotide sequences reported in this paper are available in the GenBank™, EMBL, and DDBJ databases under the accession no:  Gen Bank: EU684476.1, Gen Bank: EU519463, Gen Bank: EU668900

Keywords: Immunoscreening, Lymphatic filariasis, cDNA library, Vaccine

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