Optimization and characterization of thermostable β-glucosidase by Scytalidium thermophilum SKESMBKU01 and Humicola sp.SKESMBKU03

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S. Santosh Kumar, Edla Sujatha, Kuraganti GunaSwetha

Department of Microbiology, Kakatiya University, Warangal-506009, Telangana, India

In the present study, β-Glucosidase production from Scytalidium thermophilum SKESMBKU01 and Humicola sp. SKESMBKU03 was investigated and optimization of the cultural conditions to enhance production of enzymes has been reported. To enhance the production level of the enzyme, different cultural conditions were optimized and observed that optimum pH and temperature for β-Glucosidase production was pH-6.0 (Scytalidium thermophilum SKESMBKU01) pH-5.0(Humicola sp. SKESMBKU03) and 45oC respectively. Maximum enzyme production was recorded on 3rd day of incubation period in shake flask (100RPM) containing Mandel’s Weber medium. Among nitrogen sources, yeast extract, peptone, beef extract and malt extract were found to be the best nitrogen source for β-glucosidase production. β-Glucosidase activities are higher in media containing glucose as their carbon source (1%) followed by xylose and lactose. The organism showed maximum dry weight in cellulose as carbon sources, yeast extract and malt extract as nitrogen source, pH of 9.0-10.0 and temperature of 45oC. β-glucosidase produced by the Scytalidium thermophilum SKESMBKU01 and Humicola sp. SKESMBKU03 are highly stable at pH 8.0 and temperature of 75oC. The results indicate that β-glucosidase produced by Scytalidium thermophilum SKESMBKU01 and Humicola sp. SKESMBKU03 are more stable at high temperature and alkaline pH.

 Keywords: Scytalidium thermophilum SKESMBKU01, Humicola sp. SKESMBKU03,β-glucosidases, optimization, characterization.

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