Optimization Of Culture Conditions For Enhanced Production Of Antimalarial Artemisinin By Streptomyces Sp. Using Response Surface Methodology

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Parcha Sreenivasa Rao, P.Lakshmi and M.Lakshmi Narasu

Department of Biotechnology, NIT, Warangal, Telangana, India

Centre for Biotechnology, JNT University, Hyderabad, india

                                         

Optimization of media components for maximal biomass production of a Streptomyces sp. involved in the bioconversion of arteannuin B to artemisinin, a potential antimalarial drug has been studied by Response Surface Methodology (RSM). A 24 full-factorial central composite design was chosen to explain the combined effects of the five medium constituents, viz. malt extract, yeast extract, K2HPO4, MgCl2 and NaCl. A second order full polynomial equation was developed by applying multiple regression analysis to the experimental data. The coefficient of determination (R2) of the model was 0.8630. The linear effects of malt extract (p < 0.004156), yeast extract (p < 0.001355) and MgSO4 (p < 0.001355) and multiple effects of K2HPO4 (p < 0.005575) were found to be more significant than other factors, i.e., (p < 0.05) from the results of analysis of variance and regression of a second order model. The optimized media composition for maximal biomass production consisted of malt extract 19.1732 (g/l); yeast extract, 6.3287 (g/l); K2HPO4, 0.941 (g/l); MgSO4, 0.60895 (g/l) and NaCl, 0.53667 (g/l).  Our studies further indicate that the increase in biomass has increased the protein concentration and bioconversion activity of the crude extract of  Streptomyces sp.

Keywords: Artemisinin, Arteanuin B, Bioconversion

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