Dept. of Chemistry, Uttaranchal University, Dehradun, India

Berberis chitria Lindl leaves and roots were extracted with  four different solvents: n-hexane, ethanol (95%), methanol and  water using  soxhlet extraction method. The phytochemical screening showed the presence of alkaloids and flavanoids in root extract only. The antimicrobial activities of different extracts of plant tested against 8 pathogenic bacteria and 3 fungal strains by disc diffusion method at different concentration (100, 50, 25 mg/mL). The methanolic extract of root produced good inhibition zone against  E.coli (37mm), P. Vulgaris (48mm), Clostridium (47mm)  whereas leaves extracts of plant do not show any activity against any pathogenic bacteria. Ethanolic extract of roots active against Fungal strain of Arthrogrophis cuboida (29mm) and A. fumigatus (32mm).

 Keywords: Berberis chitria, antibacterial, inhibition zone, roots and leaves.