Preliminary study on the immunological function in Eothe-nomys miletus from Hengduan mountain region

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Yuqiu Liao , Bowen Yan , ZhengkunWang , WanlongZhu

Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming 650500

The immune functions of animals play an important role in their own survival, enabling animals to resist the invasion of foreign bodies and maintain the homeostasis of their internal environment. Eotenomys miletus is a typical representative of mammals in the Hengduan mountains of China, in order to preliminarily explore the immunological function of E. miletus, the present study took 12 E. miletusas the research object, 6 for experimental group, injected Phytohemagglutinin (PHA), 6 for the control group, injected with Phosphate-Buffered Saline (PBS), to determine the degree of swelling of the posterior foot pads, the wet organ mass and the body composition. The results showed that the PHA response decreased with the prolongation of time in E. miletus, and the 6 hours after injection reached the maximum, reaching 0.89, which gradually decreased after 6 h. And the difference in wet organ mass and body composition between the control group and the experimental group was not obvious. In conclusion, injection of PHA makes immune system respond to the interference of adverse conditions and maintain its normal survival in E. miletus. The current study can provide a theoretical basis for the subsequent study of the immunological function of E. miletus in Hengduan mountains under different conditions.