To study the biofilm formation and antibacterial activity of some medicinal plants against Streptococcus sp. isolated from diabetes type 2 patients

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Manisha Nanda, Vinod Kumar, Rajendra Prasad and Saurabh Pant

Dept. of Biotechnology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, Uttarakhand, India

Dept. of Chemistry, Uttaranchal University, Dehradun, Uttarakhand, India

Dept. of Forestry, Uttaranchal University, Dehradun, Uttarakhand, India

Type 2 diabetes patients have significantly higher plaque index and a higher prevalence and magnitude of root surface caries than non-diabetic subjects. In the present study we have isolated two Streptococcus sp. from oral samples of diabetes type 2 patients and studied the biofilm formation by these bacteria. Biofilm formation act as a defensive method during various stress conditions for some bacteria. Streptococcus mutans, the principle cariogen for dental caries, co- exist with over 500 other species of bacteria as an interactive community known as the dental biofilm (Jubai 2015). The inhibitory effect of some medicinal plants against the isolated Streptococcus sp. were also studied.  For this purpose Walnut (Juglans regia) stem bark, cinnamon (Cinnamomum zeylanicum) and clove (Syzygium aromaticum) extracts were used. The results obtained displayed that the aqueous extract of clove exhibits highest antibacterial activity against these isolated biofilm forming Streptococcus sp. C1 and Streptococcus sp. C2.

 Key words: Biofilm;antibacterial activity; diabetes type 2; Streptococcus sp.; Plant extract.

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