1. Laboratoire de Biotechnologies, Université Félix Houphouët-Boigny d’Abidjan-Cocody, Unité de Formation et de Recherches Biosciences, 22 BP 582 Abidjan, (Côte d’Ivoire),
2. Université Nangui Abrogua, UFR STA, Laboratoire de Biotechnologie et Microbiologie Alimentaire, 02 BP 801, Abidjan (Côte d’Ivoire)
3. Unité de Bio-industries, Gembloux Agro-Bio Tech, Université de Liège. 2, Passage des Déportés. 5030, Belgium
This work reports for the first time that Penicillium canescens synthesises a probable quorum sensing substance (QRS) 1-octen-3-ol. The impact of this molecule on the morphological changes of this fugus and its biosynthesis of industrial metabolites endo-β-(1, 4)-xylanase and β-galactosidase was highlighted. After seven days of culture, 11 ± 1 and 17 ± 1 µg of 1-octen-3-ol/ml were quantified in 863 and in submerged fermentation media (SFM), respectively. In solid-state fermentation (SSF), 105, 106 and 107 spores/g as initial inoculum caused the productions of 363 ± 36, 636 ± 39 and 1000 ± 38 µg of 1-octen-3-ol/g of soya oil cake, respectively. However, under growth circumstances, the level of 1-octen-3-ol was too weak (inferior to 84 µg/ml on 863 medium and 5859 µg/g of soya oil cake, respectively) to influence the growth of P. canescens and its production of endo-β-(1, 4)-xylanase and β-galactosidase. Also, the inductive conidiation effect of 1-octen-3-ol was not obvious, and so, it can’t be considered as a quorum sensing substance in P. canescens.
Keyword: Fermentation; Penicillium; Quorum sensing; Enzymes; Fungi.