In vitro Establishment of a Threatened Plant Species Adina Cordifolia

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Pratima Raypa, A.K. Verma, Ashutosh Dubey, and Salil K. Tewari

Dept. of Biochemistry, College of Basic Sciences and Humanities, G. B. Pant Univ. of Ag. & Tech., Uttarakhand, India

  Agroforestry Research Centre, G. B. Pant Univ. of Ag. & Tech., Uttarakhand, India

 

Adina cordifolia (Haldu)is tree species belonging to the family Rubiaceae. The paste of the stem bark or leaves heel deep wounds and jaundice, stomach ache, malarial fever, swelling in stomach and root is useful for dysentery and the direct seeding of Adina cordifolia failed due to low viability. Due to destructive mode of harvesting, Adina cordifolia is included in threatened species. Thus there is need to save this plant from being extinct. Adina cordifolia, apical buds were used as an explant for in vitro establishment. The explants collected in the month of March to June were surface sterilized by 5% (v/v) Tween-20 for 5 minute, 0.1% Bavistin for 5 minute, 0.1% (w/v) HgCl2 for 1 minute and 70% ethanol for 1 minute and placed on the surface of Murashige and Skoog (MS) medium containing different combination of hormone BAP and NAA. The maximum survival rate of explants were observed 66.67% at 1 mg/L BAP. The shoot length was highest in MS medium supplemented 2mg/L BAP alone and 0.5mg/L NAA alone.

                Key words: Adina cordifolia, explants,shoot length, MS medium

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