Characterization And Antagonistic Potential Of Soil Actinomycetes Against Pathogens Of Human Mycosis

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PragyaJadon, Ramendra Singh Parmar, Charu Singh and Ajay Kumar

Department of Life Sciences, ITM University, Gwalior - 475001, Madhya Pradesh, India

 

This study was conducted to isolate and identify the biologically potential actinomycetes. Total of 70 cultures were isolated from different habitat of Gwalior region, Madhya Pradesh, India. All isolated actinomycetes evaluated for their ability to inhibit human pathogens, i.e.Microsporumcanis, Microsporumgypseum, Microsporumfulvum, Tricophytonrubrum and Tricophytonmentagrophyte. All isolates were screened for their antifungal activity by agar well method against dermatophytes. After screening out of these isolates, only one isolate designated as AP-27 showed antifungal activity against Microsporumcanis, Microsporumgypseum, andTricophytonrubrum. Cultural, morphological and biochemical studies of isolate AP-27 classified it into Streptomyces spp. The 16S rRNA gene sequence of isolate AP-27 classified as Streptomyces griseus. This study proves that actinomycetes isolated from soil of Gwalior region have good antifungal activity against dermatophytes.

 

Keywords: Soil actinomycetes; Antifungal activity; Dermatophytic fungi; secondary metabolite.

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