Insilico Approach for Sinapyl Alcohol Dehydrogenase (SAD) Protein Analysis in Cedrus deodara

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Goyal, N. and Ginwal, H.S.

Genetics and Tree Propagation Division, Forest Research Institute, Dehradun Uttarakhand (India)

 

 Deodar (Cedrus deodara) is an important timber tree belongs to family Pinaceae, due to the high lignin content. It grows in the high altitude with the elevation range of 1200m to 3050m. The sinapyl alcohol dehydrogenase participates in the formation of syringyl lignin through phenylpropanoid pathway of monolignols. The protein product of SAD gene is responsible for high lignin content which provides benefit in timber industry. Here the phylogeny analysis, three dimensional protein model followed by the validation to show more emphasis on evolutionary relationship, structure and function of sinapyl alcohol dehydrogenase (SAD). The model structure showed the suitability with a member of NADP (H)-dependent dehydrogenase family which catalyzes the formation of monolignols from Populus tremuloides. The model revealed the stable confirmation during the study of atomic flexibility. In the future perspective molecular docking can be study for the response in defence mechanism against the pathogens and it will more visualize the self-defence pathway of the deodar tree.

 

Keywords: Evolutionary Relationship; Lignin Biosynthesis; Phenylpropanoid Pathway; Sinapyl Alcohol Dehydrogenase;Tertiary Structure.

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