Department of Microbiology, Sai Institute of Paramedical and Allied Science, Dehradun

           Dept. of Crop Improvement, College of Forestry, Ranichauri, Distt. Tehri, Garhwal-249199,                                                                  Uttarakhand, India

                       G. B. Pant Engineering College, Ghurdauri, Pauri, Uttarakhand, India

The polluted water from tannery effluent exposed area of Ganges River from Kanpur at various site were collected for physicochemical and microbiological analysis. Physicochemical analysis of Ganga water from Bithoor’s sites, pH was recorded as highest (8.60). D.O (8.8 mg/L) was quite optimal however chloride was high (131.24 mg/L). The B.O.D was recorded as (10.0 mg/L) whereas conductivity (0.426 mg/L) was the lowest and it has same value of salinity (0.1 ppt) like Jajmau. Sarsaiya Ghat site revealed B.O.D (13.92 mg/L), chloride values (62.66 mg/L), highest value of alkalinity (103.7 mg/L) and possess same conductivity (0.45 ppt) as in Shuklaganj. The value of soluble phosphate of Shuklaganj site was recorded as 1.465 mg/L whereas the value of total alkalinity was the lowest among all the four sites (15.25 mg/L). The pH value of Jajmausite is the lowest (7.72) whereas B.O.D and T.D.S of the sample was the highest 15.2 mg/L and 221.2 mg/L respectively. T.S was 232.0 mg/L. The chloride was recorded as (185.44 mg/L), which was the highest among all the sites. It has same value of salinity like Bithoore site. A total of thirty bacteria isolated from Ganga River, out of these five were selected for present study on the basis of their distinct morphotypic characteristics. These bacteria also tested for their potential to solubilize phosphate and metal tolerance for Chromium. All four bacterial isolates were able to tolerate 75 ppm concentration of chromium except B. Bacterial strain B was able to tolerate up to 100 ppm. Three strains had phosphate solubilizing potential. Among all the strains S exhibit high phosphate solubilizing potential and reduced the level of BOD from initial 2.52 mg/L to 2.42 mg/L content in the sewage. The present study evaluates that the identified bacteria were used to bio-remediate contaminated waste water and sewage.

Keywords:Bioremediation; Heavy Metal tolerance; Phosphate solubilization; Sewage degradation.