Immunoscreening Of Lymphatic Filariid Brugiamalayi L3 Cdna Library Reveals Novel Vaccine Candidates

  • strict warning: Non-static method view::load() should not be called statically in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/views.module on line 1113.
  • strict warning: Declaration of views_handler_argument::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_argument.inc on line 917.
  • strict warning: Declaration of views_handler_argument::query() should be compatible with views_handler::query($group_by = false) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_argument.inc on line 917.
  • strict warning: Declaration of views_plugin_pager_none::post_execute() should be compatible with views_plugin_pager::post_execute(&$result) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/plugins/views_plugin_pager_none.inc on line 69.
  • strict warning: Declaration of views_plugin_query::options_submit() should be compatible with views_plugin::options_submit($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/plugins/views_plugin_query.inc on line 169.
  • strict warning: Declaration of views_plugin_argument_default_taxonomy_tid::options_submit() should be compatible with views_plugin_argument_default::options_submit($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/modules/taxonomy/views_plugin_argument_default_taxonomy_tid.inc on line 119.
  • strict warning: Declaration of views_plugin_style_default::options() should be compatible with views_object::options() in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/plugins/views_plugin_style_default.inc on line 24.
  • strict warning: Declaration of views_plugin_row::options_validate() should be compatible with views_plugin::options_validate(&$form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/plugins/views_plugin_row.inc on line 136.
  • strict warning: Non-static method view::load() should not be called statically in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/views.module on line 1113.
  • strict warning: Non-static method view::load() should not be called statically in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/views.module on line 1113.
  • strict warning: Non-static method view::load() should not be called statically in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/views.module on line 1113.
  • strict warning: Declaration of views_handler_field::query() should be compatible with views_handler::query($group_by = false) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_field.inc on line 1147.
  • strict warning: Declaration of content_handler_field::element_type() should be compatible with views_handler_field::element_type($none_supported = false, $default_empty = false, $inline = false) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/cck/includes/views/handlers/content_handler_field.inc on line 229.
  • strict warning: Declaration of views_handler_filter::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_filter.inc on line 587.
  • strict warning: Declaration of views_handler_filter::query() should be compatible with views_handler::query($group_by = false) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_filter.inc on line 587.
  • strict warning: Declaration of views_handler_filter_search::exposed_validate() should be compatible with views_handler::exposed_validate(&$form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/modules/search/views_handler_filter_search.inc on line 112.
  • strict warning: Declaration of views_handler_area::query() should be compatible with views_handler::query($group_by = false) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_area.inc on line 81.
  • strict warning: Declaration of views_handler_area_text::options_submit() should be compatible with views_handler::options_submit($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_area_text.inc on line 121.
  • strict warning: Non-static method view::load() should not be called statically in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/views.module on line 1113.
  • strict warning: Declaration of views_handler_sort::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_sort.inc on line 165.
  • strict warning: Declaration of views_handler_sort::options_submit() should be compatible with views_handler::options_submit($form, &$form_state) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_sort.inc on line 165.
  • strict warning: Declaration of views_handler_sort::query() should be compatible with views_handler::query($group_by = false) in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/handlers/views_handler_sort.inc on line 165.
  • strict warning: Non-static method view::load() should not be called statically in /home/u809185268/domains/sciencebeingjournal.com/public_html/sites/all/modules/views/views.module on line 1113.
Prashant Kumar Singh, Vishal K Soni, Mohd. Shahab, ShailjaMisra-Bhattacharya

Division of Parasitology, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, Uttar Pradesh 226031

 

Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, M.P

Immuno screening of cDNA library has been the most preferred choice to pick up desired gene from a pool of genome.We have identified three novel immunodominant cDNA clones, BmL3-thioredoxin, BmL3-hydrolase and BmL3-Cytochrome P450 by immunoscreening of a larval stage cDNA library of Brugiamalayi. Currently there are no adulticidal drugs or vaccines available against worms that are causative agents of lymphatic filariasis. The identified clones can be potent vaccine candidates for developing prophylactic agents against the human filarial parasite. Nucleotide sequences reported in this paper are available in the GenBank™, EMBL, and DDBJ databases under the accession no:  Gen Bank: EU684476.1, Gen Bank: EU519463, Gen Bank: EU668900

Keywords:Immunoscreening, Lymphatic filariasis, cDNA library, Vaccine

application/pdf icon| PDF (1 MB) |